gastric carcinoma cell line ags  (ATCC)

Journal: International Journal of Molecular Sciences

Article Title: Gastric Inflammation Impacts Serotonin Secretion in a Mouse Model of Helicobacter pylori Vaccination †

doi: 10.3390/ijms26167735

Figure Lengend Snippet: Effect of H. pylori infection on gastric epithelial cell responses to 5-HT. ( A ) The AGS gastric epithelial cell line was co-cultured with H. pylori 26,695 for 6 h, and the expression of 5-HT receptors (top) and IL-8 (bottom) was determined by qPCR. ( B ) Pretreatment with 5-HT for 5 min did not affect the induction of IL-8 expression by H. pylori after 6 h. ( C ) The expression of HTR1A protein in AGS cells exposed to H. pylori for 6 h. HTR1A (green) was distributed in the cytoplasm and nuclear regions (arrows), nuclei (blue), and F-actin (red). ( D ) Activation of NF-κB as reflected by the nuclear translocation of the p65 subunit 0.5 h after exposure to H. pylori , with or without 5-HT (arrows). Exposure to H. pylori induced NF-κB translocation but not after treatment with 5-HT alone. 5-HT treatment did not increase H. pylori -induced NF-κB activation. ( E ) Activation of ERK as reflected by nuclear translocation in AGS cells exposed to H. pylori for 4 h. Both PMA and H. pylori exposure caused nuclear translocation (white arrows), but translocation was blocked by combined 5-HT and H. pylori (x) (representative results from three independent experiments).

Article Snippet: Gastric epithelial carcinoma cell line (AGS) cells (ATCC CRL-1739, passage 5–10) were cultured in RPMI containing 10% foetal calf serum (FCS) and penicillin/streptomycin in the presence of 5% CO 2 .

Techniques: Infection, Cell Culture, Expressing, Activation Assay, Translocation Assay

Journal: Translational Cancer Research

Article Title: Guanine monophosphate synthase-mediated nuclear and mitochondrial communication in the progression of gastric cancer

doi: 10.21037/tcr-2024-2244

Figure Lengend Snippet: GMPS knockout significantly inhibited tumor properties in AGS, MGC-803, and HGC-27 cells. (A) CCK-8 assay results show that proliferation was inhibited in the GMPS-sgRNA group. (B) The cell cloning experiments show similar results to the CCK-8 assay (stained with 0.1% crystal violet). (C) The EdU experiments show a decrease in positive cells in the GMPS-sgRNA group (stained with EdU). (D) Transwell experiments yield similar results to the EdU assay (stained with DiI). *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001. CCK-8, Cell Counting Kit-8; OD, optical density.

Article Snippet: The human gastric carcinoma cell lines AGS, MGC-803, and HGC-27 obtained from the American Type Culture Collection were maintained in RPMI-1640 medium supplemented with 10% foetal bovine serum (FBS) and penicillin-streptomycin.

Techniques: Knock-Out, CCK-8 Assay, Cloning, Staining, EdU Assay, Cell Counting

Journal: Translational Cancer Research

Article Title: Guanine monophosphate synthase-mediated nuclear and mitochondrial communication in the progression of gastric cancer

doi: 10.21037/tcr-2024-2244

Figure Lengend Snippet: JC-1 stained AGS, MGC-803, and HGC-27 cells were analyzed by flow cytometry. There were no significant differences between the Control-sgRNA group and the GMPS-sgRNA group.

Article Snippet: The human gastric carcinoma cell lines AGS, MGC-803, and HGC-27 obtained from the American Type Culture Collection were maintained in RPMI-1640 medium supplemented with 10% foetal bovine serum (FBS) and penicillin-streptomycin.

Techniques: Staining, Flow Cytometry, Control

Journal: Translational Cancer Research

Article Title: Guanine monophosphate synthase-mediated nuclear and mitochondrial communication in the progression of gastric cancer

doi: 10.21037/tcr-2024-2244

Figure Lengend Snippet: The mitochondrial ROS levels in AGS, MGC-803, and HGC-27 cells were analyzed by flow cytometry. GMPS knockout increased ROS generation in AGS, MGC-803, and HGC-27 cells. ROS, reactive oxygen species.

Article Snippet: The human gastric carcinoma cell lines AGS, MGC-803, and HGC-27 obtained from the American Type Culture Collection were maintained in RPMI-1640 medium supplemented with 10% foetal bovine serum (FBS) and penicillin-streptomycin.

Techniques: Flow Cytometry, Knock-Out